Five edible-nest swiftlet houses in Central Java were sampled for fecal specimens.The samples were obtained using a sterile container to maintain aseptic conditions.Subsequently, the samples were mixed thoroughly in a tube containing sterile saline and allowed to incubate seat tube spacer for a duration of 30 minutes.One milliliter of supernatant was placed onto Potato Dextrose Agar (PDA) with chloramphenicol (50 mg/L) and incubated at a temperature of 25 ºC for a duration of one week.
The results showed that all samples had a high percentage of Aspergillus niger (100%), Aspergillus flavus (100%), and Aspergillus fumigatus Divertor Seal (100%) isolates.The findings suggest that edible-nest swiftlet houses in Central Java can serve as a potential reservoir and conduit for fungal infection and transmission.